Function
of Spectrophotometer Parts
Exit slit:
Determines the “band pass” of the
instrument
The range of
wavelengths that pass through the exit slit and strike the reaction cuvet
The larger the exit
slit the wider the band pass
Reaction
cuvet:
Holds the reactants and are of
various types:
Single cuvets
Holds only one sample
and is removed following use.
Flow through cuvets
A sample is aspirated
into cuvet, measured, and then another sample is aspirated, washing the first one
out of cuvet.