Beer’s
Law
Definition
The absorbance of a solution is
directly proportional to the concentration of solute and length of light path.
Calculation
of unknown concentration
Cu = Au x Cs
As
As
To prove
reaction is linear:
A standard curve is prepared using
varying dilutions of a standard and the absorbance (A) or %Transmittance (%T) is plotted. A
straight line indicates linearity.