Example
of Calculation
The Doctor has ordered a hemoglobin
assay on his patient Johnnie B. Goode. When you ran the assay you got an absorbance of 0.350. The
14 gm/dL hemoglobin
standard gave an absorbance of 0.450
Cu = Au x Cs
As
As
Cu = 0.350 x 14 gm/dL
0.450
0.450
Cu = 10.9 gm/dL
Johnnie B. Goode has a
Hb = 11
gm/dL