Cytochemical
Classification-1
Myeloperoxidase stain (MPO):
Enzyme catalyzes action of H2O2 on 3-amino-9-ethylcarbazole to form black
precipitate
All neutrophils except myeloblasts.
Weakly positive in monocytes
Stains phospholipids, neutral fats,
and sterols black.
Sudan Black B stain (SBB):
As-D chloracetate:
Myeloid cells positive
Esterases (specific):
Monocytes
negative/weakly positive
Chloracetate :
Neutrophils positive
Leukemic myeloblasts
positive
Same cells positive as for MPO