Cytochemical
Classification-4
Leukocyte Alkaline phosphatase (LAP):
Hydrolyses naphthol AS-BI phosphate
to form precipitate
with fast red-violet dye
Bands & segmented neutrophils
positive
Hydrolyses naphthol AS-BI phosphoric
acid to form precipitate with diazo salts
Acid phosphatase:
Most cells are positive but if
tartrate is added only lymphocytes of Hairy Cell Leukemia (HCL) remain positive
Monoclonal antibodies to this enzyme
are added along with fluorescent tagged antibodies to detect enzyme
Termininal Deoxynucleotidyl Transferase (TdT:
Pre-B and immature T lymphocytes are
positive