Unit Notes - 1
1. How can hemolyzed specimens used for zinc protoporphyrin be used without having patient redrawn:
Centrifuge tube then remove plasma and wash red cells using 0.85% NaCl
If O.D. of Hb A2 was 0.150 and the O.D. of total Hb was 0.450, what is the % Hb A2
2. Calculation of Hb A2:
Hb A2 = O.D. of Hb A2              x  100
                 O.D. of total Hb x 5
Hb A2 = 0.150         x  100
                 0.450 x 5
Hb A2 = 6.7%