Unit
Notes - 3
5. Test of
choice based on Hb F levels:
Hb F of 20 –
40%
Hb F 0f 10 –
15%
6. Principle of Singer alkali denaturation test:
Red cell
hemolysate is incubated with potassium hydroxide which destroys all hemoglobins
except Hb F. The Hb F is then measured.
7. Principle of Radial Immunodiffusion assay for Hb F:
Agar plates
containg anti-Hb F antibodies have wells into which patient red cell hemolysate is
added and let diffuse. The diameter of each ring is proportional to conc.
Singer alkali
denaturation
Betke alkali
denaturation