G6PD
Fluorescence Test
Quantitative method:
Following reagents placed in test
tube and patient’s hemolysate added. Amount of light absorbed is directly proportional to amount of G6PD
present in serum
NADP
Glucose-6-phosphate
Reaction of assay:
Glucose-6-phosphate
+
NADP
G-6-PD
6-phosphogluconate
+
NADPH
fluoresces
Amount of fluorescence is directly
proportional to concentration of G-6-PD