Assays
for Pyruvate Kinase
Quantitative method:
Following reagents placed in test
tube:
ADP
Phosphoenolpyruvate
acid
Reaction of assay:
ADP
+
ATP
PK
Pyruvic acid
+
NADP
Pyruvic acid
Amount of fluorescence is inversely
proportionate to concentration of PK
NADH
Phosphoenolpyruvate
acid
+
LD
lactic acid
+
NAD
fluoresces