1	One Step Further Production of Monoclonal Antibodies OSF - 16 Pages 293 & 294
2	Monoclonal Abs - 2 Definition: emonoclonal antibodies are antibodies that possess a single specificity (idiotype) for a specific antigenic determinant (epitope) Alternative name: ehomogeneous antibodies Techniques used to produce monoclonal antibodies: esomatic cell hybridization (cell fusion) erecombinant DNA technique Principle of monoclonal production by somatic cell hybridization: emonoclonal antibody production is based on the fact that each member of a B lymphocyte clone (daughter cells) will express the same idiotype throughout their life span thus maintaining the identical epitope specificity
3	Monoclonal Abs - 3 Somatic cell hybridization technique: Mouse-enzyme deficient myeloma cell + B lymphocyte from mouse that has been injected with specific antigen Cell fusion Hybridoma cell Cultured in media that allows only hybridomal cells to survive Monoclonal Abs with ag specificity
4	Monoclonal Abs - 4 Recombinant DNA technique: emRNA is isolated from lymphoid tissue of donor who has been sensitized with a specific antigen eisolated mRNA is converted to dsDNA using reverse transcriptase + DNA polymerase enzymes epolymerase chain reaction (PCR) is used to amplify the gene fragments of V_H and V_L ethe amplified fragments of V_H and V_L are then injected into E. coli bacteriophage via a plasmid vector and gene fragments fuse with bacteriophage protein eeach recombinant bacteriophage contains a different combination of V_H and V_L genes thus producing antibodies of differing specificities. ethese specificities are then identified by reacting with different antigens and are then cataloged into a monoclonal antibody library
5	End of Monoclonal Abs