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1	One Step Further Molecular Fingerprinting OSF - 17 Page 323
2	Molecular Fingerprinting - 2 Application: etechniques used for the detection of polymorphism of HLA alleles Molecular techniques: eRestriction fragment length polymorphism (RFLP) esequence-specific priming (SSP) esequence-specific oligonucleotide probing (SSOP) esequence-base typing (SBT) uses Southern blotting technique identifies HLA loci that code for individual HLA antigens alternative name = Amolecular fingerprinting@ uses PCR/gel electrophoresis technique detects polymorphism at allele level uses PCR/hybridization probes to PCR products detects HLA alleles uses PCR/nucleotide sequencing of PCR products detects alleles at exact sequence level
3	Molecular Fingerprinting - 3 Restriction fragment length polymorphism (RFLP) technique: eDNA is extracted from patient=s cells eDNA is visualized using Southern blotting technique eHLA iantigens are identified by comparing patient=s band patterns to control (known) band patterns ecan be used to distinquish one individual from another
4	End of Molecular Fingerprinting