1	One Step Further Vaccine Design OSF - 3 Page 30
2	Vaccine Design - 2 Types of vaccine formulations (designs): eattenuated esynthetic antigen elive viral vector eSubunits eNaked DNA Attenuated vaccines: eFirst type of vaccines used eStill used for the following: ŽMycobacterium tuberculosis ŽSalmonella typhii ŽBordetella pertussis eshort-lived and limited protection eLive attenuated viral vaccines are more effective than are killed ones: ŽPolio vaccines ŽMeasles vaccines
3	Vaccine Design - 3 Synthetic Antigen Vaccines: eSynthesized peptides that resemble antigenic determinants (epitopes) of a particular infectious agent eThis type of vaccine made possible with developments in: Židentification of bacterial antigenic structure Žrecombinant DNA technology Židentification of specific epitopes eApplications of this type of vaccine include: ŽHepatitis B vaccines ŽHerpes simplex vaccines
4	Vaccine Design - 4 Live Viral vector vaccines: eUses a live recombinant viral vector (carrier) eGene that codes for the desired antigenic detrminants (epitopes) are inserted into a non-pathogenic virus eAltered virus is then used as the vaccine eApplications under investigation include: ŽHIV ŽHBV ŽHerpes simplex vaccines
5	Vaccine Design - 5 Subunit vaccines: eConsists of specific, defined regions of purified antigen from pathogenic organisms eThese subunits are capable of inducing a protective immune response eApplications under investigation include: ŽDiphtheria - tetanus toxin ŽBacterial polysaccharides of: Hemophilus influenzae Pneumococcus ŽHBsAg
6	Vaccine Design - 6 Naked DNA vaccines: einject only the DNA molecule (naked DNA) of pathogenic organism into host muscle tissue eMuscle cells incorporate naked DNA eMuscle cells then will synthesize antigenic structures of introduced DNA code
7	HBV vaccine - 7