Laboratory
Detection of Antigen
Methods of Detection:
˜Monoclonal
antibodies
ˆan antibody
produced against a specific epitope
ˆcan be used
to detect antigen in host
ˆcan be used
to detect antibody to antigen in host
ˆused to
detect antigens in:
Userum
Uplasma
Ucells
˜Detection of
antigen in cells
ˆmonoclonal
antibody can be tagged with a fluorescent dye which then binds to antigen
ˆa fluorescent
microscope can then be used to see antigen
