Immunoglobulin
Fragmentation - 6
Outcomes from fragmentation experiments:
eall basic Ig molecules (monomeric forms)
have 2 separate antigen-binding
sites (FAB) each containing VL and VH pair
eeach VL - VH pair serves as an independent antigen- binding site
ethe secreted form of IgM contains five
monomeric units - thus
10 separate antigen-binding sites
ethe hinge region of an Ig molecule provides
flexibility which
determines how many of the ab=s
antigen-binding sites
will bind with epitopes on antigens
ethe location and functions of the Fc portion
of the Ig are separate
from that of the FAB region
