Conditions for Maximum Reaction
•Factors that will contribute to maximum binding of antigen by antibody:
•1.specificity of antibody for antigen
•2.close proximity of antigen and antibody
•3.high affinity (forces of attraction) and high avidity (strength of binding) of antibody for antigen
•4.presence of approximately equal numbers of epitopes and idiotypes
•5.appropriate concentration of antigens and antibodies
•6.appropriate environmental conditions