Detection of Secondary Ab-Ag Reactions & Examples of Tests
•eSince the end result of this stage is
visible precipitation or agglutination, no labels are necessary for detection of end products.
•eExamples of Techniques are:
•UImmunoprecipitin
techniques
•when soluble
mulivalent antigens form insoluble Ag-Ab complexes that are not visually detected
•when Ag-Ab
complexes form the cloudiness of the solution changes
•when Ag-Ab
complexes form the light passing through the solution is scattered and the amount of light at right-angles is
measured