Introduction
to Precipitation Techniques
Definition:
Immunoprecipitation
is the formation of insoluble
Ag-Ab complexes called Aprecipitins@, when soluble antigen is mixed with it=s specific
antibody.
Factors that
must be considered when performing immunoprecipitation techniques:
Urelative
concentrations of antigen and antibodies
Uhydrogen ion
concentration (pH)
Uionic
strength of solution or reaction media
Uantibody
affinity and avidity
