Double Immunodiffusion – Technical Errors
•eAlteration of precipitin lines by:
•1.excessive condensation in wells
•2.improper filling of wells
•3.incubation in tilted position
•efalse results due to misidentification of wells
•efalse negative results due to under incubation
•efalse results due to incorrect interpretation of pattern
•einvalid controls or standards used