Single
Radial Immunodiffusion
Also called Oudin procedure:
One of the
reactants is incorporated into the medium and is in fixed position and concentration while
the other reactant diffuses
through the medium from wells cut into medium.
agar with Ab=s to Ag being tested
Standards of
differing concentrations added to wells a - d
a
b
c
d
precipitin rings
the larger the ring the greater the conc. of Std.
