Two
Methods of Determining Conc.
eEnd-Point Method (Mancini):
eKinetic Method (Fahey & McKelvey)
1.antigen
allowed to diffuse until completion - about 24 hours
2.the ring
diameter is directly proportional to concentration of antigen
3.standard curve
used to convert ring diameter to concentration
1.antigen
allowed to diffuse for limited time - about 18 hours
2.the ring
diameter is proportional to the log of antigen concentration
equivalence point is reached
equivalence point is not reached
