Clinical Applications & Errors
•Clinical Applications:
•Identification and quantification of serum proteins:
•-immunoglobulins (IgG, IgM, IgE, IgD, IgA)
•-"1-antitrypsin
•-transferrin
•-complement components (C3, C5, etc.)
•Technical errors:
•incorrect filling of wells
•specimen contamination
•wrong media or outdated media
•incorrect construction of standard curve
•incorrect incubation time or plate position
•incorrect measuring of ring size or calculations