Electrophoretic Techniques - 1
•General concept:
•By applying voltage through a support medium it is possible to separate complex protein mixtures
•Types of support media used in clinical applications:
•Upaper
•Uagarose gel
•Ucellulose acetate
•Factors influencing protein molecule migration:
•Usurface charge on molecule
•Umolecular weight of molecule
•Usize of molecule
•Umedium characteristics
•Ubuffer characteristics (pH)
•Utemperature