Electrophoretic Procedure
•
Serum, urine, or CSF is applied to support medium and voltage
is applied
•
point of
application
•
anode
(+)
•
cathode
(-)
•
a stain is added that reacts with separated proteins to form a
visible band of color
•
the strip is then placed in a densitometer which converts each
band into a peak based on intensity of color and width of band