Immunoelectrophoresis - Concepts
•Utypically performed as confirmation when a monoclonal component is detected on protein electrophoresis.
•Uallows for characterization of monoclonal proteins by identifying:
•-specific heavy-chain classes
•-specific light-chain components
•Ua two-step process that involves:
•-electrophoretic separation of proteins in serum or urine
•-immunodiffusion/precipitation of separated proteins with antiserum