IFE – General Concepts
•IFE = Immunofixation Electrophoresis
•UUsed as an alternative to immunoelectrophoresis
•UA two step procedure as is IEP.
•UDiffers from IEP in:
•\the antisera is directly overlayed onto gel instead of
• being put in a trough
•\more sensitive
•\shorter incubation time (2 hours compared to overnight)
•\more expensive and labor-intensive