IFE – General Concepts
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IFE = Immunofixation Electrophoresis
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Used as an alternative to immunoelectrophoresis
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A two step procedure as is IEP.
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Differs from IEP in:
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the antisera is directly overlayed onto gel instead of
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being put in a trough
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more sensitive
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shorter incubation time (2 hours compared to overnight)
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more expensive and labor-intensive