Light
Scattering Immunoassays
General concept:
Uwhen ag-ab
complexes are formed in a solution they form immunoprecipitates which have the ability to
scatter a light beam
that passes through the solution.
Turbidimetric
application:
U detectors
set at 180o angle measures reduction in light
Nephelometric
application:
U detectors
set at 90o angle measures increases in light
in both
techniques standard curves are constructed to convert absorbance to concentration
in
turbidimetry the absorbance is directly proportionate to concentration so std curve is linear and fewer standards needed
in
nephelimetry the standard curve is non-linear and requires calibrators
