Ureduce ionic strength of medium
Udecrease
particle surface charge
Uincrease
viscosity
Ureduce
surface charge of RBC=s
Uincrease
contact
Umaintain
temperature
Umaintain pH
LISS
22% albumin
dextran
enzyme
treated
centrifuge
IgG = 37oC
IgM = 4oC
6.7 - 7.2
Enhancement of Agglutination