Factors Affecting Agglutination - 2
•U class of antibody
•U charge of the Acarrier@ particles
•U number of epitopes
•U concentration of reactants
•U environmental factors
•generally the higher the concentrations the stronger and faster the reaction
•may get weak or negative reactions if prozone (>Ab) or postzone (>Ag) exists
•reactions enhanced by:
•-proper mixing
•-proper temperature
•-proper incubation time
•-proper centrifugation
•-proper agitation
•on previous screen