Fluorochrome Labels
•Definition:
•a compound that has the property of absorbing light of one wavelength and emitting light of another wavelength
•Examples:
•9fluorescein isothiocyanate
•9phycobiliproteins
•green light
•red light
•Characteristics of a good fluorophore for clinical use:
•9must not interfere with antigen-antibody binding
•9must maintain their stability
•9must be able to absorb excitation light in available wavelengths
•9must be able to emit light in an appropriate wavelength