Competitive
Binding Protocol - 1
Definition:
a procedure
in which labeled and unlabeled molecules (antigens or antibodies) compete for a limited number of specific binding-sites on binder or
receptor molecules.
Procedure:
9a
commercially prepared label antigen is added in excess of available antigen-binding sites on the
antibody molecule
9patient serum
containing the unlabeled antigen is then added and will compete with the labeled antigen
for antigen- binding
sites on the antibody molecule
9a separation
step is necessary to remove the unbound antigen (both labeled and unlabeled) from the test
system
9an
appropriate measuring device then determines the amount of labeled antigen left in the system (bound
to ab)
