Competitive
Binding Protocol - 2
Procedural information: continued
9if the presence of antibodies in patient
samples is to be measured
then a commercially prepared antigen is adsorbed unto a solid phase and labelled antibody and
patient sample are added
and compete for epitopes on antigen
9in the reaction phase equilibrium must be
achieved (all ag-ab complexes
have formed)
9in the separation step it is necessary to
have the ag-ab complexes
formed bound to a solid surface so they are not washed out of the system
