Sandwich
Technique
Definition:
a same as the
non-competitive binding technique for antigen detection.
Procedure:
9a
commercially prepared antibody is bound to a solid phase
9patient serum
containing the unlabeled antigen is then added and will bind with the bound antigen forming
Ag-Ab complexes
bound to solid phase
9a separation
step is necessary to remove the unbound antigen from system
9a labeled
antibody is then added in excess which will bind to the multivalent antigen sandwiching the
antigen between the two
antibodies.
9a separation
step removes unbound labeled antibody
9the amount of
labeled antibody is then measured
9the amount of
labeled antibody is directly proportional to amount of antigen in patient sample
