One-Step
Sandwich Method
Advantages:
9extremely
sensitive
One-step assay:
9a
commercially prepared antibody is bound to a solid phase
9patient serum
(antigen) and labeled antibody are both added
9an incubation
period allows reaction to occur:
9the bound
antibody and labeled antibody reacts with different epitopes on the multivalent antibody
9a separation
step removes unbound labeled antibody
9the amount of
labeled antibody is then measured
9the amount of
labeled antibody is directly proportional to amount of antigen in patient sample
