RAST
Competitive Radioallergosorbent assays:
(RAST)
also called Aallergen profile@, allergy screen@, or Aallergen-
specific IgE
quantification@
uses the
competitive binding technique where labeled IgE and
patient IgE
compete for binding sites on a specific allergen
bound to a
cellulose disc.
uses
radioisotopes as labels
amount of
labeled IgE left in system is measured using
appropriate radiation detector:
amount of
labeled IgE left in system is inversely proportional
to amount of
allergen-specific IgE in patient sample
