RIST
Competitive Radioimmunosorbent assays:
(RIST)
uses the
competitive binding technique where labeled IgE and patient IgE compete for binding sites on an
anti-IgE antibody bound
to a cellulose disc.
uses
radioisotopes as labels
amount of
labeled IgE left in system is measured using
appropriate
radiation detector:
amount of
labeled IgE left in system is inversely proportional
to amount of
allergen-specific IgE in patient sample
