EIA
•Enzyme Immunoassays: (EIA)
•uses the competitive binding technique where labeled ligand and patient ligand compete for binding sites on a ligand- specific antibody bound to a solid surface.
•may utilize homogeneous EIA
•E no separation step required
•or
•may utilize heterogeneous EIA
•E a seperation step required to seperated labeled from non-labeled
•uses enzymes as labels
•Common applications include:
•E automation of immunoassays
•E low levels of drugs detected
•E low levels of hormones detected