ELISA
•Enzyme-Linked Immunosorbent assays:
•uses the competitive binding technique where labeled ligand and patient ligand compete for binding sites on a ligand- specific antibody bound to a solid surface.
•uses enzymes as labels
•After seperating bound from free labeled and non-labeled
• ligands an appropriate substrate is added
•The amount of color complex formed is measured using a
• spectrophotometer
•The amount of color developed is inversely proportional to
• amount of ligand in patient serum