EMIT
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Enzyme-multiplied Immunoassays: (EMIT)
uses the Asandwich@ technique where patient ligand binds with sites on a ligand-specific antibody bound to
a solid surface. A labeled ligand-specific antibody is then
added which binds with antibody-antigen complex
uses enzymes as
labels
After seperating
bound from free labeled antibodies an appropriate substrate is added
The amount of
color complex formed is measured using a spectrophotometer
The amount of
color developed is directly proportional to amount of ligand in patient serum
