Chemiluminescence
•Principle:
•assays that utilize molecules that, upon being excited, emit light through chemical reactions
•Types of chemiluminescent labels:
•9Acridinium esters
•eupon oxidation they emit a Aflash@ of light
•9Luminol
•eupon oxidation they emit a Aglow@ of light
•Types of chemiluminescence assays:
•9both competitive binding and non-competitive binding reactions
•9both homogeneous and heterogeneous protocols may be used
•Clinical applications:
•9Blotting procedures
•9tumor markers
•9PSA