Immunofluorescence
- 1
Principle:
assays that
utilize molecules (fluorochromes) that emit light at a longer wavelength than the wavelength
of the absorbed light.
Types of fluorochromes:
9fluorescein
isothiocyanate
eabsorbed
wavelength = 490 nm
eemited
wavelength = 517 nm (green)
9rhodamine
eabsorbed
wavelength = 515 nm
eemited
wavelength = 546 nm (red)
9flow
cytometer
Method of measurement:
9fluorescence microscope (UV light source)