Immunofluorescence - 2
•Types of immunofluorescence assays: (IFA)
•9indirect immunofluorescence assays (IIF)
•eused to detect circulating autoantibodies in serum
•9direct immunofluorescence assays (DIF)
•eused to detect deposited autoantibodies on tissues
•Types of immunofluorescence reagents:
•9both use commercially prepared fluorescein-labeled mono- specific antibodies