Immunofluorescence
- 2
•Types of immunofluorescence assays: (IFA)
•9indirect
immunofluorescence assays (IIF)
•eused to
detect circulating autoantibodies in serum
•9direct
immunofluorescence assays (DIF)
•eused to
detect deposited autoantibodies on tissues
•Types of immunofluorescence reagents:
•9both use
commercially prepared fluorescein-labeled mono- specific antibodies