IIF - 1
•Indirect immunofluorescence assays: (IIF)
•9serum samples:
•enon-competitive binding procedure
•epatient sample (antibody) added to slide with antigen fixed to it
•ewashing step removes any unbound patient antibodies
•elabeled anti-immunoglobulin is added that will bind to ag-ab complex
•efluorescent ag-ab-labeled ab complex then measured in fluorometer
•econcentration of antibody in patient serum is directly proportional to amount of fluorescence