IIF - 2
•Indirect immunofluorescence assays: (IIF)
•9tissue samples:
•epatient tissue sample (antigen + autoantibody complexes) fixed to slide
•elabeled anti-immunoglobulin is added that will bind to ag-ab complex
•efluorescent ag-ab-labeled ab complex then analyzed using a fluorescence microscope
•ethe following observations are made:
•jpattern of deposits (granular or linear)
•jextent of staining (focal or diffuse)
•jlocation of deposits (nuclear, basement membrane, mitochondria, etc.)