Events
Occurring in Flow Cytometry
9 Light
Scatter
9 Measurement
of fluorescence
E Foreward
light scatter:
E Side light
scatter:
U measured by
in-line detector
U measured by
detector set at 90o
U measures
cell size
U measures
cell complexity
E Fluorochrome labeled monoclonal
antibodies
mixed with
sample
E amount of fluorescence is directly
proportional to
amount of
antibody on cell
