Complement
Fixation Tests - 1
•ecomplement
acts as an indicator (production of hemolysis) in detecting ag-ab reactions.
•ecomplement in
patient sample is destroyed by:
•U heating at
560C for 30
minutes
•U ag + ab +
complement in a series of dilutions are incubated together
•E stage 2 -
indicator system
•U sheep RBC=s + anti-sheep antibodies (hemolysin) are added and incubated