Complement Fixation Tests - 1
•ecomplement acts as an indicator (production of hemolysis) in detecting ag-ab reactions.
•ecomplement in patient sample is destroyed by:
•U heating at 560C for 30 minutes
•U ag + ab + complement in a series of dilutions are incubated together
•E stage 2 - indicator system
•U sheep RBC=s + anti-sheep antibodies (hemolysin) are added and incubated
•E stage 1 - test system