Cell
Separation Techniques - 2
Cell cultures
U patient
lymphocytes are stimulated using various substances to proliferate
U a labeled
DNA precursor - 3H-thymidine
is added to culture and
amount of radiocativity remaining in harvested cells is an indication of lymphocyte function
U Mixed
lymphocyte reaction:
\ used to
evaluate a patients lymphocyte response to foreign HLA antigens
\ one-way
protocol:
\ two-way
protocol:
lymphocytes
exposed to foreign HLA on lymphocytes treated so they don=t respond
both lymphocyte
populations allowed to respond
