Evaluation
of Neutrophil Function
Nitroblue tetrazolium dye reduction test
(NBT test)
U neutrophils
can reduce NBT dye via respiratory burst producing H2O2 as a
result
U failure to
reduce the NBT dye (colorless to blue- black deposits called Aformazan@) or produce H2O2 indicates metabolic defects which can lead to:
chronic
granulomatous disease
Flow cytometry
- now method of choice
Chemiluminescence
U neutrophils
emit small amounts of energy (Chemiluminescence) during respiratory burst
U luminol+latex
particles phagocytized by neutrophil and amount of light emitted can be measured using a luminator
