Histocompatibility
Testing – Procedure -1
Microcytotoxicity test for class I HLA:
U isolated
blood cells are reacted with a reagent panel of antisera (known HLA antigen specificity)
U complement
is added to test system
U hemolysis
indicates antigen-antibody complex has formed (>50% of cell lysed)
U no
hemolysis indicates no antigen-antibody complex has formed
or
U a dye may
be added (eosin or trypan blue) which the target cell will uptake if ag-ab has activated
complement (>50% uptake
dye)
