Cross-matching
Procedures
•Mixed
Lymphocyte Reaction
•Lymphocytotoxicity
via chromium-release assay
•\Donor + recipient lymphocytes are combined
•\strength of reaction is proportional to the
differences in HLA
•\donor T cells + patient serum (ab=s) + tagged anti-Human globulin allowed to flow through flow cytometer
•\crossmatch is positive when T cells show class
I HLA incompatibility