Cross-matching Procedures
•Mixed Lymphocyte Reaction
•Flow cytometry
•Lymphocytotoxicity via chromium-release assay
•\Donor + recipient lymphocytes are combined
•\strength of reaction is proportional to the differences in HLA
•\donor T cells + patient serum (ab=s) + tagged anti-Human globulin allowed to flow through flow cytometer
•\crossmatch is positive when T cells show class I HLA incompatibility