Monoclonal Abs - 3
•
Somatic cell hybridization technique:
•
Mouse-enzyme
deficient
myeloma cell
•
+
•
B lymphocyte from mouse
that has been injected with
specific antigen
•
Cell fusion
•
Hybridoma cell
•
Cultured in media
that allows only
hybridomal cells
to survive
•
Monoclonal
Abs with ag
specificity
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